Figure 2.

Effect of XWL-1-48 on growth of human cancer cells in vitro. (A) The cytotoxic effect of XWL-1-48 on growth of human cancer cells was determined by MTT assay. IC₅₀ was calculated by Prism6.0 software. The results represent the mean ± SD of three independent experiments each done in duplicate. (B) HepG2 cells were seeded in a 6-well plate, and incubated with XWL-1-48 (1, 2.5, 5 nM) or GL331 (5 nM) for 24 h. After 10–14 days, colonies (>50 cells) were fixed and manually counted. A representative of three experiments is shown. (C) Data were shown as mean ± SD of three independent experiments. ^** p < 0.01 vs. control.