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. 2017 Aug 30;7:9989. doi: 10.1038/s41598-017-10577-7

Figure 4.

Figure 4

Effect of XWL-1-48 on cellular apoptosis of HCC. (A) Treatment with XWL-1-48 (0.1, 0.3, 1 µM) and GL331 (1 µM) for 24 h, early and late apoptotic cells were detected by flow cytometry. A representative of three independent experiments is shown. (B) Apoptosis of HepG2 cells was detected by DAPI staining. HepG2 cells were treated by XWL-1-48 or GL331 for 24 h, then the cells harvested, fixed and stained with 4, 6-diamidino-2-phenylindole (DAPI). (C,D and E) HepG2 cells were incubated with XWL-1-48 (0.1, 0.3, 1 µM) and GL331 (1 µM) for 24 h, expression of Bcl2, Bax, Fas, Fas L, cleave caspase-3 and caspase-3 were determined by western blot analysis. A representative result is shown from at least three independent experiments. (F) In addition, effect of XWL-1-48 on caspase-3 activity was measured by Caspase-3 colorimetric assay. Data were shown as mean ± SD of three independent experiments, *p < 0.05, **p < 0.01 vs. control group, respectively.