Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 30;7:9597. doi: 10.1038/s41598-017-10246-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The HyHEL-10 Fv fragment changes its structure upon binding to lysozyme. (a) HSQC spectra of free and bound HyHEL-10 are shown in black and red, respectively. (b) Chemical shift differences between the free and bound forms are plotted for the V_H (top) and V_L (bottom) domains. Above each panel, the α-helix and β-strand regions are shown as open and filled boxes, respectively. Yellow and red lines represent the average and average plus one standard deviation of the chemical shift differences, respectively. (c) The crystal structure of bound HyHEL-10 (PDBID: 1C08) with residues highlighted in the same colors as in (b). The V_H and V_L domains are shown in light green and light blue, respectively. (d) The RMSD between the crystal structure of the free (PDBID: 5AYU) and bound (PDBID: 1C08) forms of HyHEL-10, which are superimposed via the V_L domain, is shown in a continuous color scheme from light blue to red, corresponding to RMSD values of 0.08 and 3.27, respectively. Crystal packing that possibly affects the RMSD values was observed in both the 5AYU and 1C08 structures particularly for residues in the crystal packing contact surface at the loops in both V_L and V_H, corresponding to the region colored in red.