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. 2017 Aug 30;7:9974. doi: 10.1038/s41598-017-09707-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTEN silencing in control PASMCs induces increased pAKT levels. Commercially available PASMCs were transfected with siRNA for PTEN or control non-targeting siRNA (scrambled siRNA), with 10% FBS used for the control group. Representative immunoblot (a) and densitometric quantification (b) of protein expression after siRNA transfection. The bars represent the mean ± SEM for n = 3 samples. **P < 0.01, ***P < 0.001 compared with the 10% FBS control group.