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. 2017 Aug 30;7:10007. doi: 10.1038/s41598-017-10270-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AtPRD1 might act bridge interaction with AtPRD3 and AtDFO. (a) The constructs expressing both the bait and the bridge proteins for Y3H assays. (b) The results of the Y3H assay. The yeast cells harboring pA-AtSPO11-1 and pB-AtPRD1 (1-388 aa)-MTOPVIB or pA-MTOPVIB and pB-AtPRD1 (1-388 aa)-AtSPO11-1 could grow well in SD/-Ade-His-Leu-Trp or SD/-Leu-Trp-His-Met media, indicating that AtPRD1 did not influence the interaction of MTOPVIB with AtSPO11-1. The yeast cells harboring pA-AtPRD3 and pB-AtPRD1 (1-388 aa)-AtDFO or pA-AtDFO and pB-AtPRD1 (1-388 aa)-AtPRD3 could grow in SD/-Leu-Trp-His-Met medium but not in SD/-Ade-His-Leu-Trp medium, indicating that AtPRD1 might function as a bridge between MTOPVIB, AtPRD3 and AtDFO. (c) BiFC assay indicated AtPRD1 might be a bridge protein to mediate the interaction of AtPRD3 and AtDFO. Bars = 50 μm.