Table 1.
The point mutation in mtopVIB-3 affected the splicing of the MTOPVIB RNAs.
| Plants | Samples | Sequencing of transcripts |
|---|---|---|
| Ler-0 | 5 (7) | correct transcript |
| Ler-1 | 2 (7) | intermediate transcripts with an extra 136-bp intron |
| mtopVIB-3-1 | 5 (13) | intermediate transcripts with an extra defective 136-bp intron |
| mtopVIB-3-2 | 4 (13) | −43 bp |
| mtopVIB-3-3 | 1 (13) | +32 bp |
| mtopVIB-3-4 | 2 (13) | +11 bp |
mtopVIB-3-1 containing 136-bp intron might translate 493 aa with 221R-K. mtopVIB-3-2 lost 43 bp up to the point mutation. mtopVIB-3-3 and -4 contained 32-bp and 11-bp intron after the point mutation, respectively. The last three cases might cause early termination of translation.