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. 2017 Aug 30;7:10001. doi: 10.1038/s41598-017-10582-w

Figure 2.

Figure 2

Deficiency of serotonin decreased collagen deposition in postoperative intra-abdominal adhesions in mice. Postoperative intra-abdominal adhesion formation was induced by caecum rubbing, and the mice were sacrificed on the 1st, 3rd and 7th days after the operation. The adhesive tissues in each group were removed and processed for analysis. (A) Representative images of picrosirius red staining showing collagen fibres in the adhesive tissues (magnification ×200); (B) Positively stained areas in the adhesive tissues using picrosirius red was calculated by ImagePro Plus 5.0 software; (C) The thickness of the collagen deposition in the adhesive tissues; (D) The hydroxyproline content in the adhesive tissues. n = 6, mean ± SEM, *P < 0.05 vs. the WT group.