Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 30;7:9984. doi: 10.1038/s41598-017-10225-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

hTau KI proteins were highly post-translationally modified and bound to microtubules. (a) 2D gel electrophoresis was performed on total protein extracts retrieved from hTau KI Drosophila heads (upper panel). hTau isovariants were detected using an anti-hTau antibody. Lambda phosphatase (λPPase) treatment of the protein extracts resulted in a drastic shift of hTau protein species towards basic pH (lower panel). (b) An in vivo microtubule-binding assay indicated that hTau proteins bound to microtubules in hTau KI Drosophila heads. Incubating protein samples with Taxol induced Tubulin polymerisation, as indicated by the increased amounts of acetyl-Tubulin retrieved in the pelleted fraction. hTau KI proteins precipitated with acetylated Tubulin in Taxol condition. The vehicle, i.e. DMSO, was used for control experiments. S: supernatant, P: pellet. Western blots were cropped in this figure; full blots are shown in Supplementary Figure 16.