Table 4.
Key salt tolerance-related genes identified from the root transcriptome of A. officinalis: The DEG sequences of A. officinalis were aligned with 4 of the published root transcriptome and microarray data that were obtained from the roots of Bruguiera gymnorhiza, rice and Arabidopsis in response to salt treatment.
| Unigene ID | Ref ID | % similarity | e-value | bit score | Gene name |
|---|---|---|---|---|---|
| Metabolic process (Up) | |||||
| Ug117296 | LOC_Os01g48960.1 | 76.29 | 0 | 693 | Glutamate synthase 1 [NADH] |
| Ug150578 | LOC_Os07g41750.1 | 76.69 | 3.00E-65 | 250 | 40S ribosomal protein S3-2 |
| Ug150734 | LOC_Os03g29460.1 | 81.61 | 7.00E-34 | 145 | 60S ribosomal protein L27a-3 |
| Ug152117 | LOC_Os01g53930.2 | 84.16 | 6.00E-20 | 99 | Hexokinase-1 |
| Ug19670 | LOC_Os11g21990.1 | 78.11 | 0 | 784 | Probable eukaryotic translation initiation factor 5-2 |
| Ug38087 | LOC_Os05g11710.1 | 75.95 | 8.00E-45 | 182 | 60S ribosomal protein L11-2 |
| Ug56565 | Bg04-15_E08 | 77.56 | 6.00E-78 | 291 | U1 small nuclear ribonucleoprotein |
| Ug62294 | LOC_Os11g06750.1 | 73.26 | 1.00E-69 | 265 | 60S ribosomal protein L3 |
| Ug7542 | Bg04-20_K13 | 80.24 | 8.00E-160 | 562 | Dihydroxy-acid dehydratase-like |
| Ug88245 | LOC_Os01g04730.1 | 73.03 | 6.00E-15 | 82 | 60S ribosomal protein L26-2 |
| Ug88349 | LOC_Os03g08020.1 | 80.62 | 6.00E-133 | 475 | Elongation factor 1-alpha |
| Ug90287 | LOC_Os07g07719.1 | 74.64 | 2.00E-44 | 180 | 40S ribosomal protein S18 |
| Ug115561 | LOC_Os11g42550.1 | 94.03 | 2.00E-21 | 102 | Probable beta-D-xylosidase 5-like |
| (Down) | |||||
| Ug112641 | LOC_Os09g07450.1 | 86.32 | 3.00E-21 | 104 | Flavonol synthase |
| Ug121433 | LOC_Os01g53900.1 | 79.13 | 0 | 1725 | Elongation factor |
| Ug127854 | LOC_Os03g08020.1 | 84.54 | 9.00E-49 | 193 | Elongation factor 1-alpha |
| Ug128815 | Bg05-08_B15 | 77.08 | 3.00E-41 | 165 | Xyloglucan endotransglucosylase |
| Ug135361 | LOC_Os03g36930.1 | 71.25 | 2.00E-26 | 121 | Eukaryotic initiation factor 4A-3 |
| Ug138854 | LOC_Os03g15780.5 | 73.51 | 9.00E-70 | 265 | Anthranilate synthase component I |
| Ug155336 | LOC_Os01g46610.1 | 77.70 | 9.00E-63 | 241 | Isocitrate dehydrogenase [NADP] |
| Ug15973 | AT4G26270.1 | 77.32 | 2.00E-100 | 364 | 6-phosphofructokinase 6 |
| Ug26981 | LOC_Os11g47980.1 | 76.02 | 5.00E-40 | 163 | Probable phosphoribosyl formylglycinamidine synthase |
| Ug48447 | Bg05-18_B13 | 99.05 | 8.00E-48 | 189 | Hypothetical protein |
| Ug86979 | LOC_Os07g37240.1 | 81.50 | 8.00E-167 | 588 | Chlorophyll a-b binding protein |
| Defense and stress (Up) | |||||
| Ug103082 | LOC_Os05g35400.1 | 77.07 | 3.00E-15 | 84 | Heat shock 70 kDa protein |
| Ug112102 | LOC_Os03g61960.2 | 73.26 | 2.00E-16 | 87 | Ferredoxin-3, chloroplastic |
| Ug124338 | LOC_Os01g72260.1 | 85.94 | 5.00E-10 | 67 | Cytochrome P450 94A1 |
| Ug128790 | LOC_Os09g39500.1 | 86.38 | 4.00E-60 | 231 | Ubiquitin-60S ribosomal protein |
| Ug150095 | LOC_Os05g38530.1 | 81.65 | 3.00E-45 | 182 | Heat shock 70 kDa protein |
| Ug150096 | LOC_Os11g47760.5 | 78.49 | 5.00E-44 | 178 | Heat shock 70 kDa protein |
| Ug150526 | LOC_Os11g26850.3 | 79.02 | 3.00E-34 | 147 | Adenosyl homocysteinase |
| Ug153937 | LOC_Os08g43640.3 | 78.22 | 8.00E-150 | 531 | Probable 26 S proteasome non-ATPase regulatory subunit 3 |
| Ug24565 | LOC_Os03g16860.2 | 84.85 | 4.00E-40 | 165 | Heat shock 70 kDa protein |
| Ug2927 | Bg04-11_J19 | 73.80 | 6.00E-62 | 237 | Monosaccharide-sensing protein 2 |
| Ug71715 | AT4G31990.4 | 79.25 | 0 | 830 | Aspartate aminotransferase |
| Ug74595 | LOC_Os02g14430.1 | 74.71 | 7.00E-12 | 73 | Cationic peroxidase 1 |
| Ug77739 | LOC_Os06g05240.1 | 77.27 | 5.00E-34 | 147 | Carboxypeptidase D-like |
| Ug83277 | Bg01-04_K24 | 80.69 | 5.00E-51 | 200 | Uroporphyrinogen decarboxylase |
| Ug9510 | LOC_Os03g16030.1 | 83.22 | 9.00E-74 | 278 | 17.3 kDa class I heat shock protein |
| (Down) | |||||
| Ug59429 | AT5G54080.2 | 76.88 | 0 | 719 | Homogentisate 1,2-dioxygenase |
| Ug103306 | LOC_Os03g16880.1 | 80.91 | 1.00E-16 | 87 | Luminal-binding protein 4 |
| Ug104404 | LOC_Os07g06890.1 | 78.10 | 1.00E-41 | 172 | D-lactate dehydrogenase |
| Ug11935 | LOC_Os06g46770.3 | 96.97 | 2.00E-07 | 56 | Ubiquitin-60S ribosomal protein |
| Ug12499 | AT5G03240.3 | 96.00 | 1.00E-16 | 82 | Ubiquitin-40S ribosomal protein |
| Ug130858 | Bg04-08_J10 | 78.49 | 6.00E-43 | 171 | Polyubiquitin 4 |
| Ug135016 | LOC_Os01g65380.1 | 100.00 | 5.00E-06 | 52 | Chaperone protein dnaK |
| Ug138511 | LOC_Os08g31030.1 | 77.90 | 1.00E-39 | 163 | Protein HOTHEAD |
| Ug2190 | LOC_Os06g48650.3 | 77.74 | 3.00E-104 | 381 | Subtilisin-like protease |
| Ug22930 | LOC_Os10g40614.1 | 79.20 | 1.00E-14 | 82 | 14 kDa proline-rich protein |
| Ug27580 | LOC_Os01g05790.1 | 73.77 | 1.00E-51 | 206 | Conserved hypothetical protein |
| Ug47022 | Bg01-06_P19 | 84.06 | 5.00E-51 | 198 | Gibberellin-regulated protein 4 |
| Ug59747 | Bg04-20_N09 | 76.97 | 4.00E-80 | 296 | Abscisic acid receptor PYL8 |
| Ug77182 | LOC_Os08g39140.3 | 73.78 | 0 | 641 | Heat shock protein 90-2 |
| Ug9466 | LOC_Os08g43390.1 | 90.48 | 2.00E-06 | 54 | Cytochrome P450 78A3 |
| Signaling (Up) | |||||
| Ug150075 | LOC_Os08g37490.1 | 77.21 | 3.00E-24 | 111 | 14-3-3-like protein GF14 kappa |
| Ug154422 | LOC_Os06g51170.1 | 74.57 | 3.00E-26 | 121 | Protein kinase APK1A |
| Ug74868 | LOC_Os05g25450.2 | 76.53 | 7.00E-36 | 152 | Receptor-like protein kinase |
| (Down) | |||||
| Ug119376 | LOC_Os04g43490.2 | 74.95 | 4.00E-59 | 230 | Casein kinase I isoform delta-like |
| Ug121629 | Bg05-07_K16 | 90.16 | 6.00E-15 | 80.5 | Probable LRR receptor-like serine/threonine-protein kinase |
| Ug24897 | LOC_Os03g20370.1 | 83.06 | 6.00E-73 | 274 | Calmodulin |
| Ug55273 | AT5G42440.1 | 85.29 | 1.00E-22 | 106 | Leucine-rich repeat receptor protein kinase |
| Ug63319 | Bg01-06_P19 | 79.37 | 9.00E-31 | 132 | Gibberellin-regulated protein 6 |
| Transporters (Up) | |||||
| Ug40195 | Bg03-06_L18 | 78.70 | 3.00E-46 | 185 | Organic Cation/carnitine transporter 7 |
| Ug6107 | LOC_Os08g08070.1 | 70.21 | 7.00E-37 | 158 | Sugar carrier protein C |
| Ug91442 | Bg04-02_L05 | 77.62 | 3.00E-93 | 340 | Lysine histidine transporter 1 |
| Ug91704 | LOC_Os04g55940.2 | 74.37 | 2.00E-113 | 411 | Vacuolar Cation/proton exchanger2 CAX2 |
| Ug29379 | LOC_Os06g43660.3 | 79.69 | 3.00E-179 | 628 | Pyrophosphate-energized vacuolar membrane proton pump |
| (Down) | |||||
| Ug86727 | AT2G01420.2 | 93.75 | 2.00E-12 | 73 | Auxin efflux carrier component 2 |
| Ug138409 | LOC_Os11g28610.1 | 77.89 | 6.00E-45 | 182 | Monosaccharide-sensing protein 2 |
| Transcription-related (Up) | |||||
| Ug113286 | LOC_Os04g51320.1 | 95.92 | 3.00E-14 | 80 | Trihelix transcription factor GT-3b |
| Ug42722 | LOC_Os05g34830.3 | 82.42 | 1.00E-56 | 222 | NAC domain-containing protein 2 |
| Ug42955 | LOC_Os04g46250.1 | 79.09 | 2.00E-12 | 75 | Ethylene-response factor 1B |
| Ug60441 | LOC_Os02g06910.1 | 76.67 | 1.00E-33 | 145 | Auxin response factor 25 |
| (Down) | |||||
| Ug69295 | Bg04-08_C14 | 83.44 | 6.00E-37 | 152 | Zinc finger CCCH domain-containing protein 69 |
| Ug29544 | AT2G22840.1 | 84.25 | 2.00E-33 | 143 | Growth-regulating factor 6 |
| Ug124037 | LOC_Os11g09690.1 | 96.97 | 2.00E-06 | 56 | Protein Mut11 |
| Ug129600 | LOC_Os06g06510.1 | 77.80 | 2.00E-63 | 243 | Histone H3.2 |
| Ug39187 | LOC_Os06g06510.1 | 78.76 | 5.00E-70 | 265 | Histone H3.2 |
| Cytoskeleton- and trafficking-related (Up) | |||||
| Ug147417 | LOC_Os12g44350.1 | 79.80 | 7.00E-35 | 147 | Actin-58 |
| Ug152164 | LOC_Os09g39500.1 | 86.38 | 5.00E-60 | 231 | Ubiquitin-60S ribosomal protein |
| Ug78218 | LOC_Os01g18050.1 | 81.49 | 0 | 741 | Tubulin beta-8 chain |
| Ug124903 | LOC_Os03g58840.1 | 76.28 | 1.00E-81 | 305 | Vesicle-associated membrane protein 725 |
| Ug26019 | AT1G10290.1 | 77.08 | 1.00E-127 | 455 | Dynamin-2A |
| (Down) | |||||
| Ug10821 | LOC_Os03g50885.1 | 79.74 | 0 | 773 | Actin |
| Ug129620 | LOC_Os03g51600.1 | 79.44 | 1.00E-64 | 246 | Tubulin alpha chain |
| Ug130905 | LOC_Os12g06660.1 | 83.36 | 7.00E-143 | 507 | Actin-1 |
| Ug130925 | LOC_Os03g61970.1 | 77.65 | 2.00E-52 | 206 | Actin-1 |
| Ug135408 | LOC_Os03g50885.1 | 85.30 | 4.00E-130 | 464 | Actin |
| Ug139265 | LOC_Os02g07060.1 | 83.49 | 3.00E-77 | 289 | Tubulin beta-7 chain |
| Ug47023 | Bg01-06_P19 | 86.67 | 3.00E-35 | 147 | Protein GAST1 |
| Uncharacterized (Up) | |||||
| Ug44132 | LOC_Os02g13970.2 | 74.70 | 5.00E-129 | 462 | Probable complex I intermediate-associated protein 30 |
| (Down) | |||||
| Ug45471 | Bg04-21_O09 | 74.38 | 2.00E-44 | 180 | Uncharacterized protein |
Column 1 shows the unigene ID, while column 2 represents the ID of the reference gene. Percent similarity between the sequences of A. officinalis and the reference plant is shown in column 3 while e-value is given in the column 4. Column 5 indicates the bit score and the gene name is given in the column 6. The genes that were present in more than one data set are highlighted in bold.