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. 2017 Aug 30;7:10061. doi: 10.1038/s41598-017-10347-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR induced we non-complementing alleles transmitted to the G1 progeny. (A) homozygous wildtype we ⁺ individual (left), homozygous mutant individual we (middle), G1 individual heterozygous for mutant we and CRISPR induced we mutation. (B) CrispRVariants plots of G1 mutant progeny of G0 targeted by we-g1 (3 sequences), targeted by we-g2 (1 sequence), we-g3 (6 sequences) or we-g2 + g3 (4 sequences).