Figure 2.

Knockout of mGluR5 suppresses neutrophil infiltration and inflammatory factor expression in brain tissues. (A) Brain sections of WT and KO mice were stained by HE at 24 h after sham operation and TBI. (B) Infiltrated neutrophils were tagged by CD177 in brain sections. Nuclei were stained with DAPI. The CD-177 positive fluorescence areas and mean density analysis were calculated by the software Image J. (C) IL-1β, TNF-α and IL-6 mRNA expression of the whole brain was detected via qRT-PCR. Data are presented as the mean ± standard error of the mean (*P < 0.05, **P < 0.01, NS indicates no significant difference; n = 5 per group).