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. 2017 Aug 30;7:10084. doi: 10.1038/s41598-017-10935-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Testing of the anti-YY1 (pS365) phosphospecific antibody. (a) Top: amino acid sequence of the phospho-peptide used to generate the novel α-YY1(pS365) antibody. Bottom: The synthetic peptide or phospho-peptide were dot blotted onto a nitrocellulose membrane and probed with the α-YY1(pS365) antibody. (b) Cold (non-radioactive) in vitro kinase assay using bacterially-expressed and purified GST-YY1 WT or S365A mutant with active Aurora A kinase. After completion, the reactions were separated by SDS-PAGE electrophoresis, transferred onto a nitrocellulose membrane and probed with α-YY1(pS365), α-YY1, and α-Aurora A antibodies, as indicated.