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. 2017 Aug 30;8:397. doi: 10.1038/s41467-017-00533-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Time course of expression of all ubiquitin-coding genes in the UBI4 repeat variants during heat shock. a Time series of UBI4 expression in the repeat variants (carrying a UBI4 gene encoding either 0, 1, 2, 3, or 5 ubiquitin units) during a sustained heat shock (HS) at 44 °C. UBI4 transcripts were measured by real-time quantitative PCR (RT-qPCR) using primers that specifically anneal to one ubiquitin moiety allowing the measurement of the transcripts from each UBI4 repeat variant rather than the total number of ubiquitin moieties per transcript. Inset shows the expression of UBI4 at time point 0 (before HS) in the repeat variants. b Time series of UBI1, UBI2, and UBI3 expression in the UBI4 repeat variants during the HS at 44 °C. Primers specific for the ribosomal protein-coding region were used to distinguish between the different genes. a, b Data are mean of 2 biological replicates (and 2 technical replicates per sample) ± SD