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14-3-3τ enhances the transcriptional activity of MEF2D. U937 cells were transfected with pMEF2Fluc (1 µg), pCMVβgal (0.5 µg) and plasmids expressing MEF2D (1 µg), Sp1 (1 µg), 14-3-3τ (2.5 µg) and 14-3-3τR56,60A (14-3-3m) (2.5 µg). Where indicated, transfected cells were treated with TSA for 12 h. The cells were lysed 48 h later and the lysates were analyzed by luciferase and β-galactosidase assay. All luciferase values were normalized to the β-galactosidase values.
