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. 2017 Aug 30;7:10028. doi: 10.1038/s41598-017-10715-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flowers of CRISPR/Cas9-mediated dfr-b mutants. The appearances of flowers (top) and stems (middle) and a schematic drawing of the meristem layers and their functions (bottom). L1: epidermal layer; L2: sub-epidermal layer; L3: internal tissues. (a) A flower and stem of #9-1, a bi-allelic-mutant plant. (b) A flower and stem of I. nil cv. Violet, an NT plant. (c) A flower and stem of #8-1, an L2 periclinal chimaera plant and a representation of the meristem layers of the L2 chimaera showing bi-allelic mutation only in the L1 layer. (d) The flower and stem of #36-2, an L1 periclinal chimaera plant and a representation of the meristem layers of the L1 chimaera showing bi-allelic mutation only in the L2 layer.