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. 2017 Aug 30;7:9982. doi: 10.1038/s41598-017-10155-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibiotics induced cd2068 gene expression in C. difficile. C. difficile 630 were grown in the presence of various antibiotics; 1 µg/ml ampicillin, 25 µg/ml cefoxitin, 1 µg/ml ciprofloxacin, 25 µg/ml cloxacillin, 10 µg/ml gentamicin, 1 µg/ml levofloxacin, 60 µg/ml roxithromycin, and 0.25 µg/ml vancomycin. The relative cd2068 gene expression was calculated as described in Methods. The error bars and the asterisks represent the standard errors and the statistical significance with the p values < 0.05, respectively.