Table 2.
Antibiotic susceptibility profiles of C. difficile 630Δerm control versus cd2068 mutant and the cd2068 mutants carrying pMTL84151 and pMTL-cd2068.
| Antibiotics | IC50(μg/ml) | Relative sensitivity | IC50 (μg/ml) | Relative resistance | ||
|---|---|---|---|---|---|---|
| Control | cd2068 mutant | cd2068 mutant + pMTL84151 | cd2068 mutant + pMTL-cd2068 | |||
| Ampicillin | 29.84 ± 3.2 | 17.13 ± 0.7 | 1.7* | 14.24 ± 0.6 | 25.56 ± 1.2 | 1.8* |
| Cefoxitin | 33.69 ± 1.3 | 20.54 ± 2.7 | 1.6* | 20.72 ± 1.1 | 46.03 ± 6.8 | 2.2* |
| Ciprofloxacin | 12.26 ± 0.5 | 7.81 ± 0.2 | 1.6* | 20.94 ± 0.6 | 34.50 ± 0.7 | 1.7* |
| Cloxacillin | 16.90 ± 0.6 | 4.76 ± 0.3 | 3.6* | 7.92 ± 0.3 | 9.73 ± 0.5 | 1.2* |
| Gentamicin | 28.60 ± 2.3 | 22.72 ± 2.7 | 1.3* | 107.83 ± 9.1 | 103.62 ± 15.3 | 1.0 |
| Levofloxacin | 7.28 ± 0.4 | 5.81 ± 0.7 | 1.3* | 6.89 ± 0.8 | 10.03 ± 0.1 | 1.5* |
| Metronidazole | 3.33 ± 0.5 | 2.37 ± 0.1 | 1.4* | 2.36 ± 0.1 | 3.23 ± 0.1 | 1.4* |
| Vancomycin | 2.01 ± 0.3 | 1.82 ± 0.2 | 1.1 | 3.38 ± 0.1 | 2.11 ± 0.1 | 0.6 |
Relative sensitivity refers to the fold difference in the IC50 values of the cd2068 mutant compared to the control cells. Relative resistance refers to the fold difference in the IC50 values of the cd2068 mutant carrying pMTL84151 compared to the cd2068 mutant carrying pMTL-cd2068. Asterisks represent the p value of less than 0.05.