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. 2017 Aug 30;7:9694. doi: 10.1038/s41598-017-07787-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Annexin V staining demonstrate CMLD-2 treatment induces apoptosis. Flow cytometric analysis shows the percentage of apoptotic and necrotic cells (Q1: necrotic; Q2: late apoptotic; Q3: early apoptotic; Q4: Live cells) at 24 h and 48 h in CMLD-2 -treated cells. (A) H1299; (B) A549; (C) MRC-9. Induction of apoptosis was greater in CMLD-2 -treated H1299 and A549 cells than in CMLD-2 -treated MRC-9 cells. (D) Bar graphs represent the percentage of apoptotic cells at 24 h and 48 h after CMLD-2 treatment. Cells treated with cisplatin (CDDP) served as positive control for each cell line. Error bar denotes SD; NS not significant; *p < 0.05; **p < 0.001.