Figure 5.

Estimation of the PSII excitonic connectivity in paired PSII-LHCII supercomplexes. (a) Chlorophyll a fluorescence induction curves (i.e., the OJIP transient) plotted on a logarithmic time scale (up to 1 s) for the paired PSII-LHCII supercomplexes prepared at 1.25 mg mL⁻¹ in the sucrose gradient buffer and diluted 1,000-fold either in the sucrose gradient buffer (control, black line) or in a similar salt-free buffer (no salts, red line). The excitation light intensity was 2,400 μmol photons m⁻² s⁻¹, with an emission peak at 630 nm. (b) The initial 300 µs from the OJIP transient in panel (a) replotted on a linear time scale. The normalized O–J phase of the OJIP curve is labeled W (solid line), and the theoretical exponential curve corresponding to the unconnected system is labeled W_E (dashed line). For calculation of W and W_E see Supplementary Table 2. (c) Plot of W_E – W using the values from panel (b). Graphs display the mean values ± standard deviations of six replicates.