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. 2017 Aug 30;7:9972. doi: 10.1038/s41598-017-10605-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bayesian maximum clade credibility (MCC) phylogeny of N9 gene sequences of waves 1 to 5. Viruses isolated in the Yangtze River Delta, Pearl River Delta, and other regions are highlighted in red, blue, and green, respectively. BEAST tree of H7N9 viruses estimated using NA gene sequences. Pink shadow indicates Clade I of H7N9 viruses; light blue represents Clade II. Figure 4A, time of most recent ancestor (TMRCA) of Clade II; Fig. 4B, TMRCA of the entire tree. Figure 4C, TMRCA of Clade I. Figure 4D, TMRCA of the Clade I-A lineage. Figure 4E, TMRCA of the Clade I-B lineage. Figure 4F, TMRCA of the lineage containing H7N9 viruses detected in avian or humans with HP characteristic cleavage site.