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. 2017 Aug 30;7:10053. doi: 10.1038/s41598-017-10227-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of ILC2 in IL33tg mouse cornea. (a) Gating strategy for analysis of FSC^lowSSC^lowCD45⁺Lineage(Lin)⁻Sca-1⁺ST2⁺ ILC2 cells. Note that ILC2 cells are Thy1.2⁺ and GATA3⁺. The numbers indicate the percentage of cells in each gate. (b) The proportion of Sca-1⁺ ST2⁺ ILC2 from the corneas of 20- to 30-week-old WT and IL33tg mice (Tg) determined by flow cytometry. Values indicate the percentage of ILC2 of Lin⁻ lymphocytes. Data are expressed as means ± SEM (n = 3) *P < 0.0001 (two-tailed t-test). (c,d) The intracellular flow cytometry for IL-5 and IL-13. The cells were gated into CD45⁺Lin⁻ fraction (c) and CD45⁺Lin⁻Sca-1⁺ST2⁺ fraction (d). The numbers indicate the percentage of cells in each quadrant. Similar results were obtained in two (a,b) or three (c,d) independent experiments.