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. 2017 Aug 30;7:10022. doi: 10.1038/s41598-017-10733-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron microscopy showing the epithelium of the trachea. (A) The normal trachea revealed pseudostratified columnar ciliated epithelium. (B) Higher magnification of the normal trachea showed cilia. (C) Higher magnification of the normal trachea clearly revealed the ciliary structure. (D) The neo-trachea showed stratified squamous epithelium with scanty cilia. (E) Higher magnification of the neo-trachea indicated scanty but undoubtedly present cilia. (F) Higher magnification of the neo-trachea revealed ciliary structures.