Table 1.
Smoking and smoking withdrawal effects on frontal lobe expression of glial and neuronal genes
| mRNA | F-ratio | p-value |
|---|---|---|
| Immature Oligodendroglia | ||
| CNP | 7.35 | 0.01 |
| GAL-C | 4.05 | 0.05 |
| Nestin | 11.32 | 0.003 |
| PDGFR-α | 14.94 | 0.0012 |
| PROM | 1.97 | N.S. |
| Vimentin | 0.065 | N.S. |
| Mature Oligodendroglia | ||
| MAG-1 | 8.68 | 0.0068 |
| MBP | 7.290 | 0.011 |
| MOG | 3.56 | 0.067 |
| PLP | 6.21 | 0.017 |
| RPAIN | 44.25 | < 0.0001 |
| RTN4 | 27.37 | 0.0001 |
| ST8 | 10.06 | 0.004 |
| Neuroglial Markers | ||
| CSPG4 | 2.90 | 0.10 |
| GFAP | 12.13 | 0.002 |
| GPD1 | 3.19 | 0.084 |
| GPD2 | 2.51 | N.S. |
| GSTP1 | 5.05 | 0.029 |
| NCAM | 5.19 | 0.028 |
| NTRK2 | 9.65 | 0.005 |
| Transcription Factors | ||
| FOXO1 | 1.05 | N.S. |
| FOXO4 | 22.87 | 0.0003 |
| NKX2-2 | 2.01 | N.S. |
| NKX6 | 15.73 | 0.001 |
| Olig1 | 14.80 | 0.001 |
| Olig2 | 8.33 | 0.007 |
| PAX6 | 34.69 | < 0.0001 |
| SOX9 | 0.06 | N.S. |
Frontal lobe RNA was analyzed using a targeted PCR array to examine CS effects on oligodendroglial/white matter myelin-associated gene expression. Data were analyzed by one-way ANOVA; the corresponding F-ratios and P-values are tabulated. The post hoc Tukey test was used to further identify statistically significant specific inter-group differences. Those results are shown within individual panels of Figs. 2–5. See Supplementary Table 1 for abbreviations and gene functions.