Figure 5.

ONC201 is effective in myeloma cells resisted to the commonly used chemo-therapeutic drugs. Chemo-resistant myeloma cell lines and their respective wild-type cells were cultured with different doses of ONC201 for 72 hours. There were no significant difference in the cell viability of MM cells between (A) dexamethasone-sensitive MM.1S and -resistant MM.1R, (B) bortezomib-sensitive OPM2 and -resistant OPM2 BZR, (C) carfilzomib-sensitive MM.1S and -resistant MM.1 R10R, and (D) carfilzomib-sensitive RPMI8226 and -resistant RPMI8226 CR. (E) Western blot analysis shows the protein levels of Bim in bortezomib-sensitive OPM2 and -resistant OPM2 BZR, and carfilzomib-sensitive MM.1S and -resistant MM.1 R10R. The levels of GAPDH served as protein loading controls. Results shown are representative of five independent experiments.