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. 2017 Aug 23;284(1861):20171393. doi: 10.1098/rspb.2017.1393

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — The expression of IFA38 of the wild-type and duplicate strains before and after evolution in YPD + 7% ethanol media. The strains were evolved in (a) YPD, (b) YPD + 7% ethanol and (c) YP + 2% glycerol. The bar charts show the IFA38 expression at the start of the evolution experiment (black bars) and after evolution at 500th generation (grey bars). Error bars are from three technical replicas for each of the five independent biological samples. Relative normalized fold expression was calculated by using ΔΔCt method and ACT1 was used as a reference gene.