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. 2017 Aug 23;284(1861):20171393. doi: 10.1098/rspb.2017.1393

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — Detection of loss of the engineered IFA38 duplicate via analytical PCR. The presence and loss of IFA38 duplicate was confirmed by PCR. (a) A diagrammatic view of the original gene (red) and engineered copy (blue) of IFA38 on the chromosome and the position of the primers (a,f,c,g; black arrows) used for PCR. (b) 1.5% (w/v) agarose gel representing the duplicate colonies of ancestral and glycerol-evolved strains confirmed by primers a + f giving a product of expected band size of 1008 bp. (c) 1.5% (w/v) agarose gel representing the duplicate colonies of ancestral and glycerol-evolved strains confirmed by primers c + g giving a product of expected band size 1032 bp.