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. Author manuscript; available in PMC: 2017 Aug 31.
Published in final edited form as: Anal Biochem. 2012 Dec 27;435(1):35–43. doi: 10.1016/j.ab.2012.12.011

Fig. 3.

Fig. 3

(A) A transcription reaction mixture containing AN59-M1 and AN59-M2 was purified on a preparative PAGE column. The electrophoresis was run at 150V for 20 hours. The elution was driven by a peristaltic pump at a speed of 1 ml/minute, and was pooled at 4 ml/fraction, as monitored by UV absorption (see Methods). (B) T ractions containing AN59-M1 and AN59-M2 were examined on a 10% native PAGE. The “marker” on the gel was an AN59-M2 sample previously purified using a slab PAGE gel.