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. Author manuscript; available in PMC: 2017 Aug 31.
Published in final edited form as: Anal Biochem. 2012 Dec 27;435(1):35–43. doi: 10.1016/j.ab.2012.12.011

Fig. 4.

Fig. 4

Effect of Mg2+ concentration, i.e., 0 mM, 1 mM and 5 mM as labeled, on the elution of AN59-M2. A sample was prepared in Phase A buffer and was heated at 50 °C for 10 minutes before injection. The linear gradient contained two sections. The first section was from 30% to 60% Phase B in 115 minutes. The second section was from 60% to 100% Phase B in 65 minutes. The flow rate was kept at 0.5 ml/minute.