Figure 4. DDX3X is closely associated with caprin-1 and PABP1 at the leading edge of migrating MRC5 fibroblasts.

(A) MRC5 cells were seeded on collagen 1-coated coverslips to a density of 15 000 cells/cm² and incubated for 45 min to allow for formation of lamellipodia. Processing of samples for immunofluorescence analysis by confocal microscopy, Z-sectioning and deconvolution analysis was as described in the Materials and Methods section. In addition to detection of the nucleus and actin cytoskeleton, staining was carried out for eIF4E and endogenous DDX3X. Scale bar is 20 µm. (B) MRC5 cells were seeded, processed and analysed as described in (A). In addition to detection of the nucleus and actin cytoskeleton, staining was carried out for PABP1 and endogenous DDX3X. Scale bar is 20 µm. (C) MRC5 cells were seeded, processed and analysed as described in (A). In addition to detection of the nucleus and actin cytoskeleton, staining was carried out for caprin-1 and endogenous DDX3X. Scale bar is 20 µm.