Figure 8. Sialyltransferase overexpression increases GluK2 molecular weight but does not alter desensitization.

A, western blot showing MW of myc‐GluK2a with and without sialyltransferase overexpression. Myc‐GluK2a was expressed alone or with ST‐3 in HEK293T/17 cells, myc‐GluK2a was detected by immunoblotting with an anti‐myc antibody. B, representative current traces from control HEK293T/17 cells expressing only myc‐GluK2a (con) and from cells where myc‐GluK2a was co‐expressed with either ST‐3 or ST‐6. Grey bar indicates glutamate (10 mm) application. Amplitudes are scaled. C, quantification of glutamate‐evoked current desensitization and amplitude from myc‐GluK2a alone and ST‐3 or ST‐6 co‐expressing HEK293T/17 cells. Error bars indicate the SEM. Statistical significance is indicated: ^** P < 0.01, ^*** P < 0.001. [Color figure can be viewed at wileyonlinelibrary.com]