Figure 5. Characterization of the signalling pathway underlying the increase of I _SO in dLGN INs.

A, I–V relationships of the OxoM‐sensitive currents in the presence of different drugs (as indicated; DAQ, DAQ B1; IRS, IRS‐1‐Y608P; β‐est, β‐estradiol). B, mean I _SO vs. time plot illustrating the strongly enhanced I _SO increase in the presence of DAQ B1 (n = 8). C, summary bar graph illustrating the maximal I _SO change during the application of OxoM. Hatched bars indicate that M_1/3AChR antagonists were present during the application of OxoM. D, examplar traces of voltage clamp recordings in the absence (black trace) and in the presence of OxoM (blue trace) when 50 μm PIP₃ was added to the pipette solution. E, exemplar I–V relationship of the OxoM‐sensitive current with PIP₃ added to the pipette solution. F, mean I _SO vs. time plot illustrating the strongly enhanced and prolonged I _SO increase in experiments in which PIP₃ was added to the pipette solution (blue symbols, n = 7) in comparison to the control effect (black symbols, as in Fig. 2 B). Note that muscarinic receptor stimulation is still required to cause a detectable change in I _SO.