Table 1.
Urinary excretion of DON and DON metabolites profiles differs by sex. DON (1 mg/kg bw) was administered i.p. to mice. Urine was collected and analyzed by LC–MS/MS.
| % Cumulative DON Dose | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Time | 2 h | 4 h | 8 h | 12 h | 24 h | |||||
| Sex | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ |
| DON | 11.1 ± 0.4 | 20.4 ± 7.5 | 32.9 ± 7.0 | 50.4 ± 4.6 | 35.9 ± 6.9 | 53.8 ± 4.5 * | 37.1 ± 6.9 | 54.9 ± 4.3 * | 37.3 ± 6.9 | 55.2 ± 4.3 * |
| DON-3-GlcA | 7.6 ± 4.8 | 7.1 ± 2.7 | 25.0 ± 4.9 | 20.0 ± 2.2 | 27.9 ± 4.4 | 21.5 ± 2.2 | 28.9 ± 4.4 | 22.0 ± 2.1 | 29.1 ± 4.4 | 22.1 ± 2.1 |
| DON-8,15-hk-8-GlcA | 2.2 ± 1.4 | 2.4 ± 0.9 | 7.4 ± 1.4 | 6.7 ± 0.7 | 8.3 ± 1.3 | 7.3 ± 0.7 | 8.6 ± 1.3 | 7.4 ± 0.7 | 8.7 ± 1.3 | 7.4 ± 0.7 |
| iso-DON-8-GlcA | 0.9 ± 0.6 | 1.1 ± 0.4 | 3.1 ± 0.6 | 3.1 ± 0.3 | 3.6 ± 0.6 | 3.5 ± 0.3 | 3.9 ± 0.6 | 3.6 ± 0.3 | 4.0 ± 0.6 | 3.7 ± 0.3 |
| DON-3-sulfate | ND | 0.2 ± 0.1 * | ND | 0.5 ± 0.1 * | ND | 0.5 ± 0.1 * | ND | 0.5 ± 0.1 * | ND | 0.6 ± 0.1 * |
| DON-15-sulfate | ND | 0.2 ± 0.1 * | ND | 0.6 ± 0.1 | ND | 0.6 ± 0.0 | ND | 0.6 ± 0.1 * | ND | 0.6 ± 0.1 * |
| Total | 21.7 ± 13.1 | 31.1 ± 11.5 | 68.4 ± 13.9 | 81.2 ± 7.8 | 75.7 ± 13.1 | 87.2 ± 7.6 | 78.5 ± 13.2 | 89.1 ± 7.3 | 79.1 ± 13.1 | 89.6 ± 7.2 |
Data are mean ± SEM (n = 8). Values for DON-8,15-hk-8-GlcA and iso-DON-8-GlcA are estimated based on the peak areas of the DON-3-GlcA quantifier transition. Asterisk indicates significant difference from male at time point (p < 0.05).