Table 5.
Mouse and human liver microsomes catalyze DON glucuronidation in a sex-specific manner.
| DON Glucuronide Formation (pmol/min/mg protein) | |||||||
|---|---|---|---|---|---|---|---|
| Sex | Species | DON (µM) | DON-3-GlcA | DON-15-GlcA | iso-DON-8-GlcA | iso-DON-3-GlcA | DON-8,15 -hk -8-GlcA |
| ♂ | Mouse | 5 | 529 ± 239 | 72 ± 3 | 37 ± 1 | 33 ± 8 | 33 ± 8 |
| ♀ | Mouse | 5 | 279 ± 269 * | 35 ± 0 * | 18 ± 0 * | 18 ± 0 | 18 ± 0 |
| ♂ | Mouse | 20 | 1760 ± 309 | 210 ± 29 | 145 ± 22 | 108 ± 19 | 114 ± 17 |
| ♀ | Mouse | 20 | 1068 ± 31 | 212 ± 4 | 125 ± 5 | 90 ± 5 | 84 ± 4 |
| ♂ | Human | 5 | 15 ± 15 | 35 ± 0 | ND | ND | ND |
| ♀ | Human | 5 | ND | 35 ± 0 | ND | ND | ND |
| ♂ | Human | 20 | 44 ± 0 | 224 ± 2 | ND | ND | ND |
| ♀ | Human | 20 | 44 ± 0 | 231 ± 14 | ND | ND | ND |
Data are mean ± SEM (n = 3/rep). Asterisk indicates significance from male at treatment level (p < 0.05).