Table 6.
Optimized SRM parameters on the 6500+ QTrap LC–MS/MS system. Product ions are given in the order quantifier (quant) before qualifier (qual) *.
| Analyte | Ret. Time (min) | Precursor Ion (m/z) | Ion Species | DP (V) | Product Ions (m/z) | CE (eV) | Ion Ratio (qual/quant) | LOD of Quant ** (ng/mL) |
|---|---|---|---|---|---|---|---|---|
| DON | 5.85 | 355.1 | [M+CH3CO2]− | −50 | 59.1/265.1 | −38/−18 | 0.24 | 0.7 |
| DOM-1 | 7.01 | 339.1 | [M+CH3CO2]− | −50 | 59.1/249.1 | −40/−17 | 0.36 | 0.6 |
| DON-3-sulfate | 4.98 | 375.1 | [M−H]− | −125 | 345.1/247.1 | −36/−38 | 0.80 | 0.2 |
| DON-15-sulfate | 4.87 | 375.1 | [M−H]− | −110 | 96.9/247.1 | −38/−38 | 0.06 | 0.03 |
| DOM-3-sulfate | 5.70 | 359.1 | [M−H]− | −125 | 96.9/329.1 | −38/−34 | 0.15 | 0.1 |
| DOM-15-sulfate | 5.83 | 359.1 | [M−H]− | −125 | 96.9/79.9 | −28/−118 | 0.48 | 0.06 |
| DON-3-GlcA | 5.36 | 471.1 | [M−H]− | −100 | 113.1/265.1 * | −35/−38 | 0.38 | 0.6 |
| DON-8,15-hk-8-GlcA | 4.50 | 471.1 | [M−H]− | −100 | 113.1/265.1 | −35/−38 | 0.12 | 0.5 |
| i-DON-8-GlcA | 2.71 | 471.1 | [M−H]− | −100 | 441.1/113.1 | −25/−35 | 0.47 | 0.4 |
| i-DON-3-GlcA | 5.72 | 471.1 | [M−H]− | −100 | 441.1/113.1 | −25/−35 | 0.13 | 0.4 |
| DONS 1 | 2.26 | 377.1 | [M−H]− | −130 | 79.9/331.0 | −98/−52 | 0.04 | 0.08 |
| DONS 2 | 2.82 | 377.1 | [M−H]− | −105 | 80.9/79.9 | −68/−98 | 0.15 | 0.08 |
| DONS 3 | 3.64 | 377.1 | [M−H]− | −130 | 79.9/347.0 | −98/−36 | 0.40 | 0.8 |
| DOMS 2 | 3.93 | 361.1 | [M−H]− | −25 | 80.9/249.1 | −65/−30 | 0.09 | 0.06 |
* In addition, the transition to 441.1 (CE −30 eV, ratio transitions to 441.1/113.1:0.20) was monitored; ** Limit of detection of the quantifier transition.