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(A) pH dependent hydrolysis of the peptides was monitored at pH 2, 4, 7.4, 9 and 12 using analytical RP-HPLC following a 24 h incubation at 37 °C. Reduced PaurTx-3 was used as a control. Data points are relative to amount of peptide at pH 4 and error bars are ± SE for n = 3; (B) comparisons of the analytical RP-HPLC traces of the peptides at pH 4 and pH 12 are shown and (C) MALDI-MS spectra for HwTx-IV, PaurTx-3 and SgTx-1 at pH 12 are also shown.
