Table 2.
Sequences of primers used in real time polymerase chain reaction RT-PCR.
| Gene | Sequence 5′—3′ | Amplicon Length (bp) | Accession Number |
|---|---|---|---|
| p21 | F: GAAGAGAAACCCCCAGCTCC R: TGACTGCATCAAACCCCACA |
241 | XM_003365840.2 |
| p53 | F: TACTCCCCTGCCCTCAACAA R: AGGAATCAGGGCCTTGAGGA |
252 | U37120.1 |
| Bax | F: TTCCGACGGCAACTTCAACT R: GGTGACCCAAAGTCGGAGAG |
218 | XM_005596728.1 |
| PINK1 | F: GCACAATGAGCCAGGAGCTA R: GGGGTATTCACGCGAAGGTA |
298 | XM_014737247.1 |
| Parkin | F: TCCCAGTGGAGGTCGATTCT R: CCCTCCAGGTGTGTTCGTTT |
218 | XM_005608126.2 |
| GAPDH | F: GATGCCCCAATGTTTGTGA R: AAGCAGGGATGATGTTCTGG |
250 | NM_001163856.1 |
| iNOS | F: GACAAGCTGCATGTGACATC R: GCTGGTAGGTTCCTGTTGTT |
325 | NM_001313922.1 |
| TNF-α | F: ACAGAAAGCATGATCCGCGA R: CTTGGTGGTTTGCTACGACG |
295 | NM_013693.3 |
| IL-6 | F: GAGGATACCACTCCCAACAGACC R: AAGTGCATCATCGTTGTTCATACA |
141 | NM_001314054.1 |
| β-Actin | F: CGACGATGCTCCCCGGGCTGTA R: CTCTTTGATGTCACGCACGATTTCCCTCT |
574 | NM_007393.5 |
F: sense primer; R: antisense primer; bp: base pair. p21: Cyclin Dependent Kinase Inhibitor 1A; p53: Tumor Suppressor p53; Bax: Bcl-2 associated X protein; PINK1: PTEN Induced Putative Kinase 1; Parkin: Parkin RBR E3 Ubiquitin Protein Ligase (PARK2); GAPDH: Glyceraldehyde-3-Phosphate Dehydrogenase; iNOS: inducible Nitric Oxide Synthase 2; IL-6: Interleukin 6; TNF-α: Tumor Necrosis Factor-α; β-Actin: PS1TP5-Binding Protein 1.