Table 1.

In vitro anti-thrombotic assessment of freeze dried and reconstituted fractionated and pooled abalone extract (AEC pool 4) using prothrombin time (PT) and activated partial thromboplastin time (aPTT) assays performed in rat plasma.

Sample Descriptions	SP (µg/mL)	Time (s)	INR
PT
Rat plasma (Before freeze drying)	0	9.7 ± 0.02	1.0
	50	12.1 ± 0.08 *	1.2
	100	13.3 ± 0.04 *	1.4
	150	15.2 ± 0.01 **	1.6
Rat plasma (After freeze drying)	0	9.1 ± 0.01	1.0
	50	11.2 ± 0.06 *	1.2
	100	12.4 ± 0.08 *	1.4
	150	14.8 ± 0.06 **	1.6
aPTT
Rat plasma (Before freeze drying)	0	31.4 ± 0.3	1.0
	5	39.2 ± 0.7 *	1.2
	10	50.1 ± 0.2 **	1.6
	20	71.5 ± 0.3 ***	2.3
Rat plasma (After freeze drying)	0	30.4 ± 0.01	1.0
	5	38.4 ± 0.2 *	1.3
	10	52.0 ± 0.2 **	1.7
	20	73.8 ± 0.03 ***	2.4

Note: Statistical significance determined using a one-way ANOVA with Dunnett’s Multiple Comparison Test compared to saline control with * p < 0.05, ** p < 0.01 and *** p < 0.001. SP stands for sulphated polysaccharide concentrations and INR stands for international normalised ratio.