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. 2017 Aug 1;15(8):242. doi: 10.3390/md15080242

Figure 3.

Figure 3

Bacterial rDNA reappears in axenic A. carterae cultures over time. The presence of bacterial 16S ribosomal gene (rDNA) was determined in axenic A. carterae maintained in culture with and without antibiotics. DNA was isolated from axenic cultures of A. carterae Hulbert (CCMP 1314, Bigelow National Center for Marine Algae and Microbiota) and tested for bacterial contamination by amplication of DNA using two universal primers to bacterial 16S ribosomal RNA gene; 27F (5′-AGAGTTTGATCMTGGCTCAG-3′) and Univ1492R (5′-GGTTACCTTGTTAC GACTT-3′) [50,51]. Amplicons were fractionated by 1% agarose gel electrophoresis, visualized by ethidium bromide staining. Images were generated by ChemiDocTMTouchTM (Bio-Rad, Hercules, CA, USA). Lanes 1 and 2: axenic culture, Lanes 3 and 4: the same culture after 4 months in culture without antibiotics; Lanes 5 and 6: the same culture after four months without antibiotics, followed by four months with KCS; Lanes 7 and 8: the same culture after four months in culture without antibiotics followed by three months in PS.