Figure 8.

5-hydroxycyclopenicillone (1) protects against H₂O₂-induced neuronal death in a concentration-dependent manner in SH-SY5Y cells. (A): SH-SY5Y cells were treated with 1 or vitamin C (VitC) at indicated concentrations. After 2 h, cells were exposed to 200 μM of 30% H₂O₂ (in H₂O). MTT assay was used to measure cell viability after 24 h of H₂O₂ exposure. Data are expressed as the percentage of control and are presented as the mean ± SEM of three separate experiments; * p < 0.05 and ** p < 0.01 vs. H₂O₂-challenged group (ANOVA and Tukey’s test). (B): SH-SY5Y cells were administrated with 1 for 2 h, and exposed to 200 μM H₂O₂. After 24 h, cells were examined by Fluorescein diacetate (FDA)/ propidium iodide (PI) double staining.