FIGURE 3.

Results for radiolabeling, binding affinity, and cancer cell line selection. (A) ⁸⁹Zr-DFO-AMG102 radiochemical purity by radio-iTLC, showing > 99.5% radiochemical purity after purification by PD10 column (free ⁸⁹Zr elutes at ∼100–125 mm). (B) Binding assay comparing modified DFO-AMG102 with unmodified AMG102. (C) Western blot results from MET/HGF-producing U87MG cells, MET-producing MKN45 cells, SKOV3 ovarian cancer cells for reference, and LNCAP cells as a low-MET-expressing reference. (D) Results from an ELISA assay for human HGF showing amount of HGF produced by select cell lines in spent medium.