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. 2017 Aug 30;2(4):e00340-17. doi: 10.1128/mSphereDirect.00340-17

TABLE 1 .

Primers used for molecular characterization of genetically manipulated L. donovani by PCR-based analysis

Primer no. Primer name Sequence
1 Primer 1 5′ TGTAGAAGTACTCGCCGATAGTGG 3′
2 Primer 2 5′ ACTCGGAACTTGGCAGAGTGTGCAC 3′
3 Primer 3 5′ CGCAGCTATTTACCCGCAGGACAT 3′
4 Primer 4 5′ TGGACGGGCTCCAGAGAGAATTCAGG 3′
5 Primer 5 5′ ATAGCGTTGGCTACCCGTGATATTGC 3′
6 Primer 6 5′ AACACGGCGGCATCAGAGCAGCCGATTG 3′
7 Primer 7 5′ ATGGTCAGGGTGTTCCCCTGCTGTAG 3′
8 Primer 8 5′ TACGGAGCTCTTCGAGGGACGACAT 3′
9 ZeoFP 5′ ATGGCCAAGTTGACCAGTGCCGTTCC 3′
10 ZeoRP 5′ TCAGTCCTGCTCCTCGGCCACGAA 3′