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. 2017 Aug 30;17:68. doi: 10.1186/s12896-017-0388-8

Fig. 1.

Fig. 1

Transformant verification. a Scheme for expression of vgb in Mortierella alpina ATCC 32222; b PCR analysis of transformants of Mortierella alpina. The PCR product corresponding to VHb is 441 bp (Lanes: M, 5000 bp marker; 1, negative control, wild-type strain ATCC 32222; 2, transformant VHb-20; 3, positive control, plasmid pBIG-CBXB-VHb; 4, transformant VHb-10); c CO binding spectra of cell extracts from original strain ATCC 32222 and transformant VHb-20