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. Author manuscript; available in PMC: 2017 Aug 31.
Published in final edited form as: Cell Rep. 2017 Aug 8;20(6):1372–1384. doi: 10.1016/j.celrep.2017.07.038

Figure 1. Genetic interactions between dNab2 and dfmr1.

Figure 1

(A) Schematic of the GMR>dNab2 screen. GMR-Gal4 overexpression (o/e) of dNab2 from the dNab2EP3716 allele leads to a rough eye phenotype that was enhanced by 14 and suppressed by 30 of the 200 candidate alleles. (B) Adult eyes from control (GMR-Gal4/+), dNab2 o/e (GMR-Gal4/+ ;dNab2EP3716/+), dNab2 o/e+dfmr1 heterozygote (GMR-Gal 4/+; dfmr1Δ50/dNab2EP3716 and GMR-Gal4/+;dfmr1Δ113/dNab2EP371), dNab2 o/e with dfmr1 RNAi (GMR-Gal 4/+;UAS-dfmr1RNAi/dNab2EP3716) and dfmr1 heterozygote (GMR-Gal4/+ ;dfmr1Δ50/+) adult females. (C) Negative geotaxis behavior of 5-day old control (elavC155-Gal4), pan-neuron dNab2 RNAi (elavC155-Gal4,UAS-dNab2RNAi), pan-neuron dNab2 RNAi+dfmr1 heterozygote (elavC155-Gal4,UAS-dNab2RNRi ,dfmr1Δ113M/+), or dfmr1 heterozygote (elavC155-Gal4,dfmrΔ113/+) flies. Data represent % of flies reaching the cylinder top at each time point. Each genotype represents ≥10 independent trials (10 flies/trial). Error bars=SD. (C) Anti-Fas2 stained wildtype (wt; isogenic precise excision pex41 of the element used to create dNab2ex3), dNab2 null (dNab2ex3/ex3 ), dfmr1 null (dfmr1Δ50/Δ50), dNab2 null with one copy of dmr1 (dNab2ex3 ,dfmr1Δ50/dNab2ex3, +), dfmr1 null lacking one copy of dNab2 (dNab2ex3 ,dfmr1Δ50/+,dfmr1Δ50), or trans-heterozygote (dNab2ex3,dfmr1Δ50/+,+) brains. Penetrance of (D) α-lobe or (C) β-lobe defects in the same genotypes as C with individual lobes counted as discrete events (≥24 brains per genotype). *p=4.8×10 and **p=1.5×10 (Chi square test).