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. Author manuscript; available in PMC: 2017 Aug 31.
Published in final edited form as: Cell Rep. 2017 Aug 8;20(6):1372–1384. doi: 10.1016/j.celrep.2017.07.038

Figure 6. Effect of dFMRP/FMRP loss onPAT length.

Figure 6

(A) Schematic of extended poly(A) tail length (ePAT) assay using linker PCR amplification of futsch PAT and the TvN control fragment (12 adenosines, “A12”) from control, dNab2 null (dNab2ex3, dNab2ex3) or dfmr1 null (dfmr1Δ50/Δ50) heads. Size standards indicated (A200/A300). Right panel=densitometry trace of the PCR products. (B) Bulk PAT length among total RNAs harvested from adult heads of the same genotypes in A. Short and long exposures (with size “ladder”) are shown, along with densitometry traces of each lane normalized for band intensity. (C) Bulk PAT length in N2a cells treated with ZC3H14, Fmr1, or scramble siRNAs and accompanying densitometry trace. Sizes are indicated. Boxed region highlights elongated PATs in the ~400A size range in ZC3H14 and Fmr1 siRNA cells. Western confirmation of siRNA knockdown is shown.