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. 2017 Aug 15;6:e27960. doi: 10.7554/eLife.27960

Figure 4. The mitochondrial proteins HtrA2/Omi and Endonuclease G are required for all-or-none SG death.

(A) SG cysts (dotted outlines) in OmiΔ1/OmiDf1 mutant testes containing individual Lysotracker-positive SGs (arrows). Hoechst 33342 (blue), FM 4–64 (red), Lysotracker (green). Bar: 10 µm. (B) Number of Lysotracker-positive SGs in each 16-SG cyst in OmiΔ1/OmiDf1, OmiΔ1/+, and wild type testes. Black line, mean. n ≥ 43 cysts per dose. (C) 16-SG cyst (dotted outline) in EndoGMB07150/Df(3R)BSC699 testes containing individual Lysotracker-positive SGs. Hoechst 33342 (blue), FM 4–64 (red), Lysotracker (green). Bar: 10 µm. (D) Number of Lysotracker-positive SGs in each 16-SG cyst in EndoGMB07150/Df(3R)BSC699 and EndoGMB07150/+ testes. Black line, mean. n ≥ 37 cysts per dose.

Figure 4.

Figure 4—source data 1. Number of dying SGs per 16-SG cyst in control, Omi and Endo G mutant testes in response to irradiation.
elife-27960-fig4-data1.xlsx (22.1KB, xlsx)
DOI: 10.7554/eLife.27960.015