Figure 7. Calcineurin A splicing during PN development in different tissues.

(A) UCSC Genome Browser displays of calcineurin A alternative exons (Ppp3ca ex13, Ppp3cb ex10a, Ppp3cb ex13, and Ppp3cc ex10a) side-by-side with RT-PCR of RNA from PN2 and PN28. (B) RT-PCR comparing inclusion of calcineurin A alternative exons in diverse adult tissues (BioChain tissue array mRNA).

Figure 7—figure supplement 1. Alignment of calcineurin A alternative exons.

(A) The protein sequences of the three mouse calcineurin A paralogs are aligned. (B) Diagrams aligning calcineurin A mRNAs and proteins showing relative positions of known protein domains and alternatively spliced regions. (C) RT-PCR of calcineurin A exons during postnatal development of muscle groups with differing fiber type contributions: gastrocnemius (blue), soleus (purple), and flexor digitorum brevis (yellow). Gastrocnemius contains 6%, soleus contains 37%, and FDB contains 13% slow fiber type (type I)(Katz et al., 2010; Close, 1972). N = 3 adult mice or three pooled litters for PN9. Displayed are mean with SD bars.