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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Bioorg Med Chem Lett. 2017 Jul 8;27(17):4165–4170. doi: 10.1016/j.bmcl.2017.07.019

Figure 5.

Figure 5

Activation of BSIH by H2O2 in PBS analyzed by LC-MS. LC traces of (a) reaction mixture of BSIH (100 μM) with H2O2 (5 mM) after 24 h (b) SIH (100 μM) (c) salicylaldehyde (SAL, 100 μM) (d) isoniazid (INH, 100 μM) in PBS, pH 7.4 at 254 nm. The peak eluted at 13 min in (a) has a corresponding m/z value of 242.1, matching that of authentic SIH in (b). The peak eluted at 12 min in (a) gives no specific m/z value on MS, but with identical retention time of authentic salicylaldehyde in (c). The peak eluted at 2 min has a corresponding m/z value of 138.1, consistent with authentic isoniazid in (d).