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. Author manuscript; available in PMC: 2017 Aug 31.
Published in final edited form as: Angew Chem Int Ed Engl. 2015 Nov 17;55(1):169–173. doi: 10.1002/anie.201507546

Figure 2. Optimization of MFSNPs for simultaneous delivery of genes and proteins.

Figure 2

a) A 3D profile of gene transfection (pEGFP) and transduction performance (BSA-Cy5 and HRP-RhB) of three categories of MFSNPs produced by DDG. Black dots represent 125 data points observed for 1st category: MFSNPs; Blue dots (125 data points) for 2nd category: RGD-MFSNPs; Red dots (125 data points) for 3rd category: RGD/TAT-MFSNPs. b) Confocal micrographs that reflect transfection/transduction outcomes with RGD/TAT-MPSNP of high (★) delivery performance. c) A colorimetric assay using o-Dianisidine (3,3′-dimethoxybenzidine) (50 mM) as chromogenic substrates and H2O2 as an oxidizer to quantify intracellular enzymatic activity of HRP delivered by the RGD/TAT-MFSNPs. d) The time-dependent in vitro co-delivery performance of the optimal RGD/TAT-MFSNPs based on fluorescent signal quantification associated with EGFP expression and colorimetric readouts correlated to HRP activity, respectively.