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. 2017 Aug 15;18(8):1525. doi: 10.3390/ijms18081525

Figure 3.

Figure 3

A Spearman correlation analysis between mRNA expression and methylation of RPRM CGIs of primary breast tumors and cell lines. (A) A significant inverse correlation was observed between mRNA expression and methylation of RPRM CGIs in paired clinical samples (n = 18) (p < 0.05); (B) meanwhile, in the BC cell lines, the Spearman correlation was not significant (p = n.s); (C) correlation between qMSP and qPCR assay in paired clinical samples (n = 13) was not significant (p > 0.05); (D) in cancer cell lines, an increase of percentage-methylated relative (PMR) RPRM was observed frequently with a downregulation of RPRM mRNA in ERα-negative cells; BT-20 and MDA-MB-231. In contrast, MCF7 shows upregulation of RPRM mRNA without methylation of the RPRM promoter region. However, no significant correlation was observed (p > 0.05).