Figure 6.

Effects of 26S proteasomes on oxidized or unoxidized bovine serum albumin (BSA) (BSAox or BSAunox, respectively). The arrow indicates the position of BSA band in each lane. Oxidized and unoxidized BSA were incubated at 37 °C for 24 h with or without C. hamatus or D. labrax 26S proteasome. The reaction mixtures were subjected to SDS-PAGE and the results were compared with those previously reported, showing the degradation pattern of oxidized BSA after incubation with T. bernacchii 26S proteasome. The analyses were conducted in triplicate on three different protein preparations.